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change in Electroacupuncture duration alters the jaw-opening reflex dagree

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Abstract


The purpose of this study was to investigate the effect of various electroacupuncture duration induced by acupuncture point-Zusanli (S36) electric stimulation on inhibition of amplitude of digastric electromyogram (dE \¢¥IG) evoked by noxious electric stimuli around the mental foramen. Experiments were conducted on adult rats (SD) anesthetized with intraperitoneal sodium pentobarbital (EntobarTM ) in an initial dose of 50 mgikg and treated with atropine sulfate. Maintenance doses of 4.5 mg/kg/h were given through a cannula in the femoral vein using a constant infusion pump. A pair of stimulating electrodes were inserted for noxious stimuli around the mental foramen. An irritant electric stimuli pulse (0.2 Hz, 0.1 ras duration) was produced with an intensity of about 1.5-2 times threshold for evoking the dEMG. The anterior belly of the digastric muscle was exposed and a pair of 0.1mm wire electrodes were inserted for dEMG recording. The magnitude of the dEMG was estimated by averaging the size of about 10 superimposed dEMGs recorded on an oscilloscope. Acupuncture point stimulation on Zusanli (2 Hz, 250 gs, biphasic pulse, 2 V) was delivered by Dental Electronic Anesthesia (3M, U.S.M. For periods of electronic stimulation of 10, 20, and 30min, the amplitudes of dEMG were measured on the oscilloscope and on the monitor connected to the amplifier. The rate of inhibition of the amplitudes of dEMG were analysed using percentages.
The following results were obtained : The digastric electromyogram was decreased to 73.4% of that in the control set after 10 min electroacupunture stimulation (Group I The digastric electromyogram was decreased to 77.1% (10min), 54.0.% (20min) of that in the control set after 20minutes of electroacupunture stimulation (Group 11). The digastric electromyogram was decreased to 73.3% (10min), 61.9% (20min), 76.2% (30min) of that in-the control set after 30 min of electroacupunture stimulation (Group III). From these results, it may be that in the electroacupuncture stimulation on the Zsusnali resulted in a reduction of amplitude of dEMG and that the most effective electro-acupuncture stimulation period was 20min.

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